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KMID : 0379520030190010013
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한국독성학회지
2003 Volume.19 No. 1 p.13 ~ p.19
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Gene Expression of GX-12, a New Naked DNA Vaccine for HIV Infection, in Reproductive Organs
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Park Seul-Min
Kang Kyung-Koo
Lee Dong-Sup
Park Jae-Hoon
Sohn Yong-Sung
Kim Chae-Young
Kim Byong-Moon
Kim Won-Bae
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Abstract
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GX-12 is a naked DNA vaccine developed by the DongA Pharmaceutical Co. Ltd. and Genexine for the treatment of HIV infection. This study was peformed to evaluate the biodistribution and expression of GX-12 mRNA in gonadal tissues, and to investigate the histopathological changes after the repeated intramuscular injection. GX-12 (400 mutextrm{g}/head) was injected into the left anterior tibialis once a week for four weeks. On day 1, 5, 15, 30 and 45 after the final administration, gonadal tissues (testes, epididymis, seminal vesicles, penis, prostate glands, ovaries, vagina, uterus) and the injection site (muscle) were harvested and examined for the expression of mRNA by RT-PCR. In addition, histopathological examination was peformed at each time point. At the injection site, mRNA expression of GX-12 was detected only at early time points (1 ~ 15 days after injection) but not thereafter. However, in gonadal tissues, mRNA expression was not identified at all time points both in male and female rats. There were no histopathological changes in all reproductive organs and muscle. Based on these results, it is unlikely that the plasmid DNAs of GX-12 was distributed to- and expressed in gonadal tissues, suggesting that the chance of germline integration and transmission is negligible.
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KEYWORD
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GX-12, HIV, DNA vaccine, Expression, Reproductive organ
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